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HPLC Grade Water

Methods of Water Purification

Step 1) Reverse Osmosis

Reverse osmosis is a molecular filtering technique in which water is
pushed through a semipermeable membrane. Organic molecules,
bacteria, other particulate debris, and 90 % to 97% of all ionized
and dissolved minerals are removed by the membrane.

Step 2) Ion Exchange

Ion exchange is a method of removing ions from water to generate
deionized water. Deionization is achieved by resin polymers that
exchange hydrogen (H+) and oxygen hydrogen (OH) ions for the
contaminants present in the ionized state of the water. Cation
exchanger, anion exchanger and a mixed-bed resin exchanger can
produce water with a specific resistance of more than 18 MΩ/cm.

Step 3) Ultraviolet photo-oxidation

UV photo-oxidation with wavelengths of 185 nm and 254 nm can
disrupts the DNA of live bacteria. In addition, photo-oxidation of
organic molecules lowers the total organic content (TOC) below 5 ppb.

Step 4) Ultrafiltration

Remove small contaminants (20-30 kDa) that are not removed by
filtration.

Applications

Version ANA

  • Ion Chromatography
  • High Performance Liquid Chromatography (HPLC)
  • Liquid Chromatography - Mass Spectrometry (LC-MS)
  • Graphite Furnace Atomic Absorption Spectrometry (GFAAS)
  • Inductively Coupled Plasma Spectrometry (ICP)
  • Inductively Coupled Plasma -Mass Spectrometry (ICP-MS)
  • Gas Chromatography - Mass Spectrometry (GC-MS)
  • TOC analysis
  • Ultra-trace inorganic and organic analysis
  • Standard and buffer solutions preparation

Version BIO

  • Polymerase chain reaction (PCR)
  • Immunochemistry (ICC)
  • Cell culture
  • Clinical analysis
  • 2-Dimensional electrophoresis
  • Monoclonal antibody production
DescriptionANABIO
Ultrapure water, 2.5L glass bottleUPW-A2UPW-B2
Ultrapure water, 4L glass bottleUPW-A4UPW-B4